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KMID : 0877219970010010060
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Journal of Korean Epilepsy Society
1997 Volume.1 No. 1 p.60 ~ p.70
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A Study on the Effects of Single and Repeated Electroconvulsive Shocks on Neurons and Astrocyes in Hippocampus of the Rats
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Kwon Oh-Young
Hwang Se-Jin
Chung Ho-Sam
Lim Byeong-Hoon
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Abstract
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Objectived : Electroconvulsive therapy (ECT) is an effective treatment for some disorders including depression. While definitive structral damage of brain induced by ECT is still controversial evidences of neuronal damage were reported by many authors 14 has been also suggested that neuronal activation by various manners including electroconvulsive shock (ECS), could alter the astroglial gene expression. To investigate the effects of ECS on the neurons and astrocytes of hippocampus, we obserbed the changes in morphology and the immunoreactivity of glial fibrillary acidic protein(GFAP) and S-100 protein, the specific markers for astrocytes.
Subjects and Methods : Experimental animals were treated with single or repeated (once/day for 10 days) ECS (100 v. AC, for 0.9sec) and sacrificed at 1 day or 10 days after last ECS. Luxol fast blue-cresyl violet stain, GFAP and S-100 immunohistochemistry were performed.
Result : 1) No histological and immunohistochemical changes were observed in rats treated with singel ECS. 2) Neuronal atrophy and nuclear pyknosis of hippocampal pyramidal cells and dentate granular cells were observed at 1st day after repealed ECS, but these findings were not found at 10th day after repeated ECS. 3) Increase of GFAP immunoreactivity in dentate gyrus, and S-100 protein immunoreactivity in dentate gyrus and CAl area of hippocampus were observed at 1st day after repeated ECS, but these findings were not found at 10th day after repealed ECS.
Conclusion : It is consequently suggested that repealed ECS may transiently induce the morphologic change of neurons and increase of GFAP and S-100 protein immunoreactivity, the latter may be related with neuroprotection in hippocampus of the rats
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KEYWORD
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Electroconvulsive therapy, Hippocampus, Morphologic change of neurons, GFAP and S-100 protein immunoreactivity, Neuroprotection
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